Info-enriched characterization of mobile populations. Morphological information from visuals can increase to the richness of flow cytometry info. One example is, the determine displays an if not traditional apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in Just about every populace to expose morphologically unique characteristics. These insights could not are obtained from multiplex staining alone.
As samples are obtained on the Attune CytPix Flow Cytometer, the large-pace brightfield camera captures and retailers pictures of detected situations at a amount approximately six,000 photos per second, depending upon the flow fee and picture dimension. For greater flexibility, Attune Cytometric Software package enables you to modify the picture capture frequency as necessary and select a certain gate for impression capture, if essential.
(B) RAW 264.seven macrophages are often annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The number of singlets available for Examination improves when the user trains and employs a far more correct design.
Find new insights into your experimental details by visualizing populations employing merged fluorescence and morphology information. Our software package interface wizards empower downsampling, concatenation (merged information) and dimensionality instruments which have been developed into the software for an intuitive, streamlined facts Evaluation expertise.
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Listed here, a qualified consumer has gated singlets confidently. Just after evaluating the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of greater than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow effectiveness, and include robotic integration for optimum throughput.
Our scientists will style panels in your case as many times you would like—gratis, no product purchase needed.
The Attune NxT cytometer is the responsible workhorse instrument your lab can trust in for reliable and trustworthy overall performance. The Attune CytPix product contains a significant-speed brightfield digicam and AI-pushed image Investigation software program, with modern upgrades like machine Mastering resources and an intuitive consumer interface, enabling enhanced information precision, accuracy, and a lot quicker insights into sample biology.
Buyers can perspective occasion photos in true-time to verify sample top quality, optimize protocols, and document the morphology of each and every cell inhabitants discovered for Evaluation. Imaging more info seize also benefits from acoustic concentrating, which really helps to placement the cells to ensure a sharp, centered impression is obtained.
Sound waves allow precise mobile alignment, resulting in Outstanding data top quality and ~10X faster sample flow fees.
Software note: Automated impression Assessment reduces user-to-user variability in flow cytometry gating techniques
Leverage the strength of Innovative technological innovation and innovation to speed up your discoveries. Believe in inside the Attune Flow Cytometer family members to provide the reliability, velocity, and precision that set your do the job aside.
Adaptable payment prepare from Agilent Fiscal Methods allow you to obtain the important instrumentation you need for instant undertaking, or to develop your lab.
Gating on these extended parameters lets you promptly and accurately discover populations of curiosity and make sure gating strategy with little or no handbook evaluation. The Attune application gives usage of state-of-the-artwork equipment Studying Investigation instruments within just an expertly intended consumer interface, making it exceptionally simple to use.
Because the mobile line experienced Earlier been analyzed over the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and were able to document the microbial infection a minimum of five times previously.
Agilent Value Guarantee ensures you not less than 10 years of instrument use through the day you buy. Or else, We'll credit score you While using the residual worth of the procedure towards an upgraded model.
What appeared to be a homogeneous population is definitely extra varied—an Perception that needs to be thought of when interpreting final results.